BAC Sequencing – Lolium perenne

BAC Sequencing – Lolium perenne

Aim: DNA sequencing and bioinformatic assembly of bacterial aritificial chromosome (BAC) inserts derived from Lolium perenne.

UK-Aberystwyth: Research and development services and related consultancy services

2013/S 004-004292

Contract notice


Directive 2004/18/EC

Section I: Contracting authority

I.1)Name, addresses and contact point(s)

Ibers, Aberystwyth University, Gogerddan, Ceredigion, Wales
For the attention of: Mr Cameron Pope
SY23 3EB Aberystwyth
Telephone: +44 1970 823015
Fax: +44 1970 823017

Internet address(es):

General address of the contracting authority:

Further information can be obtained from: The above mentioned contact point(s)

Specifications and additional documents (including documents for competitive dialogue and a dynamic purchasing system) can be obtained from: The above mentioned contact point(s)

Tenders or requests to participate must be sent to: The above mentioned contact point(s)

I.2)Type of the contracting authority

Other: University
I.3)Main activity

I.4)Contract award on behalf of other contracting authorities

The contracting authority is purchasing on behalf of other contracting authorities: no

Section II: Object of the contract

II.1.1)Title attributed to the contract by the contracting authority:

BAC Sequencing – Lolium perenne.
II.1.2)Type of contract and location of works, place of delivery or of performance

NUTS code UKL1

II.1.3)Information about a public contract, a framework agreement or a dynamic purchasing system (DPS)

The notice involves a public contract
II.1.4)Information on framework agreement
II.1.5)Short description of the contract or purchase(s)

Project Title: BAC sequencing – Lolium perenne
Project Description
Aim: DNA sequencing and bioinformatic assembly of bacterial aritificial chromosome (BAC) inserts derived from Lolium perenne. Background: Two bacterial aritificial chromosome (BAC) libraries have been developed from Lolium perenne genomic DNA using HindIII and BstY1 restriction to generate the inserts. These have been cloned into a standard BAC cloning vector. An estimate of the average cloned insert size = 125kb. A minimum tiling path (MTP) of c. 30000 BAC clones has been identified and will be rearrayed into 384-well microtitre plates prior to project commencement. The clones targeted for sequencing will be a subset of this MTP and the number of clones to be targeted will be determined partly on the basis of the tendered costings of interested service providers.
Requirement 1: To generate DNA ‘shotgun’ sequence coverage of individual BAC clones to a pre-defined sequencing depth within the constraints of a chosen DNA sequencing technology.
Requirement 2: To bioinformatically generate and deliver assembled DNA sequence contigs which can be related back to individual or pooled BAC clones.
Requirement 3: To describe flexible pricing structures and data generation pipelines dependent on the final number of BAC clones to be targeted, the depth of sequence coverage to be generated and any other relevant technical or bioinformatic considerations.
Requirement 4: To provide the raw sequencing data on hard disks or using equivalent suitable media.
Requirement 5: To retain back-up copies of the raw sequencing data for a minimum period of 12 months after the initial delivery of the raw sequencing data to Aberystwyth University.
Requirement 6: To provide detailed protocols of the relevant sequencing and bioinformatic pipelines on request.
Community Benefits do not apply to this contract.
II.1.6)Common procurement vocabulary (CPV)


II.1.7)Information about Government Procurement Agreement (GPA)

The contract is covered by the Government Procurement Agreement (GPA): no

This contract is divided into lots: no
II.1.9)Information about variants

Variants will be accepted: no
II.2)Quantity or scope of the contract
II.2.1)Total quantity or scope:

DNA sequencing and bioinformatic assembly of bacterial artificial chromosome (BAC) inserts derived from Lolium perenne.
Estimated value excluding VAT: 200 000 GBP
II.2.2)Information about options

Options: no
II.2.3)Information about renewals
II.3)Duration of the contract or time limit for completion

Section III: Legal, economic, financial and technical information

III.1)Conditions relating to the contract
III.1.1)Deposits and guarantees required:
III.1.2)Main financing conditions and payment arrangements and/or reference to the relevant provisions governing them:
III.1.3)Legal form to be taken by the group of economic operators to whom the contract is to be awarded:
III.1.4)Other particular conditions
III.2)Conditions for participation
III.2.1)Personal situation of economic operators, including requirements relating to enrolment on professional or trade registers
III.2.2)Economic and financial ability
III.2.3)Technical capacity
III.2.4)Information about reserved contracts
III.3)Conditions specific to services contracts
III.3.1)Information about a particular profession
III.3.2)Staff responsible for the execution of the service

Section IV: Procedure

IV.1)Type of procedure
IV.1.1)Type of procedure

IV.1.2)Limitations on the number of operators who will be invited to tender or to participate
IV.1.3)Reduction of the number of operators during the negotiation or dialogue
IV.2)Award criteria
IV.2.1)Award criteria

The most economically advantageous tender in terms of the criteria stated in the specifications, in the invitation to tender or to negotiate or in the descriptive document
IV.2.2)Information about electronic auction

An electronic auction will be used: no
IV.3)Administrative information
IV.3.1)File reference number attributed by the contracting authority:

IV.3.2)Previous publication(s) concerning the same contract

IV.3.3)Conditions for obtaining specifications and additional documents or descriptive document

Payable documents: no
IV.3.4)Time limit for receipt of tenders or requests to participate

30.4.2013 – 12:00
IV.3.5)Date of dispatch of invitations to tender or to participate to selected candidates
IV.3.6)Language(s) in which tenders or requests to participate may be drawn up

IV.3.7)Minimum time frame during which the tenderer must maintain the tender
IV.3.8)Conditions for opening of tenders


Aberystwyth University.

Section VI: Complementary information

VI.1)Information about recurrence

This is a recurrent procurement: no
VI.2)Information about European Union funds

The contract is related to a project and/or programme financed by European Union funds: no
VI.3)Additional information

Aberystwyth University will be conducting this procurement exercise through the Value Wales e-Tendering portal. This can be found at All information may be downloaded and returnes through this channel. Tenders or Requests to Participate must be sent to

Please note that this text reflects information we have produced in good faith with a view to helping economic operators to
‘get to grips’ with the e-tender software. The following information should not be considered as a definitive guide and any questions whatsoever concerning the software and the submission of your bid should be discussed with bravosolution helpdesk (details at foot).
Welcome to this etender opportunity.
If you have not used the Bravo Solution etender portal before, you are advised to download the supplier quickstart guide from the attachment section. If you have any queries or questions concerning the tender documentation, you can send these through the portal messaging system.
Please note that this tender comprises a selection of attachments which you can download to read and, typically, three ‘envelopes/questionnaires’ (qualification/technical/commercial response sections).
These ‘envelopes/questionnaires’ are areas where you will find the tender questions you need to answer, typically by attaching responses and/or completing drop down selection boxes or empty fields. Consistent with the text in our advertisement, you will need to ‘reply’ to this ITT in order to gain access to the various envelopes/questionnaires so you can read the questions requiring attention.
If you are intending to submit a response, please ensure you have completed all required actions well before the closing date and time. The ‘read me first’ section in the qualification response envelope/questionnaire contains some helpful notes and reminders for you, in this regard.
You will need to make sure you have answered all mandatory questions (i.e. anything marked with a red *) and also uploaded any general attachments that you also wish to bring to our attention.
If you do not complete all required ‘inline’ attachments and/or dropdown/option menus and/or empty fields and/or any other mandatory options which we have used (denoted by the red * in each case) in the qualification, technical and commercial envelopes/questionnaires (as appropriate) then, as we understand it (we cannot see the bidders side of the system, to protect tender intergrity) the software will not allow you to submit your bid.
If required, please seek assistance by contacting the Helpdesk direct (operated by BravoSolution):-
Freephone: 0800 368 4850
Fax 020 7080 0480

Mike Smith
Procurement Manager
Aberystwyth University
Buy4Wales Reference Number: 33206

VI.4)Procedures for appeal
VI.4.1)Body responsible for appeal procedures

Finance Office, Aberystwyth University
Old College, King Street
SY23 2AX Aberystwyth
Telephone: +44 1970622027
Internet address:

VI.4.2)Lodging of appeals

Precise information on deadline(s) for lodging appeals: The University will incorporate a minimum 10 calendar day standstill period at the point information on the award of the contrcat is communicated to tenderers. This period allows unsuccessful tenderers to seek further debriefing from the University before the contract is entered into.
Applicants have two working days from notification of the award decision to request additional debriefing and that information has to be provided in a minimum of 3 working days before expiry of the standstill period. Such additional information should be requested from the address in section 1.1.
If an appeal regarding the award of a contract has not been successfully resolved the Public Contracts Regulations 2006 (SI 2006 No 5) provide for aggrieved parties who have been harmed or are at risk of harm by a breach of the rules to take action in the High Court ( England, Wales and Northern Ireland). Any such action must be brought promptly (generally within 3 months).
VI.4.3)Service from which information about the lodging of appeals may be obtained

Finance Office, Aberystwyth University
Old College, King Street
SY23 2AX Aberystwyth
Telephone: +44 1970622027
Internet address:

VI.5)Date of dispatch of this notice:3.1.2013